Firebird Diagnostics LLC

SAMRS^TM

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SAMRS^TM

Self Avoiding Molecular Recognition Systems: SAMRS^TM

Easy test design. Dozens of targets. Expand/change assays at will.

Problem

To amplify DNA/RNA, large amounts of DNA in large numbers are added to samples as primers and probes. Unfortunately, these large amounts often interact with each other, causing the assay to collapse.

With natural DNA, the need to prevent reactions between primers constrains multiplex assay design and manufacturing even at low levels of multiplexing. With more than ~20 targets, the multiplex loses robustness, and then collapses entirely.

Solution

Modify bases in DNA primers so they bind only to the desired DNA target, not to each other.

Impact

Many assays demonstrate the value of SAMRS

Assays with dozens of targets
Multiple with speed and sensitivity of a single target
Single-tube multiplexed panels streamline workflow
Fast, inexpensive multiplex modification for emerging pathogens
Wide manufacturing tolerances; less product rejection

Chemistry behind SAMRS^TM

SAMRS bases (a, t, g, c) resemble standard bases (A, T, G, C), but strategically omit hydrogen bond units.

SAMRS a pairs with natural T, SAMRS t pairs with natural A, SAMRS g pairs with natural C, and SAMRS c pairs with natural G. However, SAMRS a does not pair with SAMRS t, and SAMRS g does not pair with SAMRS c.

Publications

SAMRS c and g Bases

The pairing pattern of standard C/G bases with SAMRS c/g bases

SAMRS c and g Bases

A simplified view of standard C/G bases with SAMRS c/g bases

SAMRS a and t Bases

The pairing pattern of standard A/T bases with SAMRS a/t bases

SAMRS a and t Bases

A simplified view of standard A/T bases with SAMRS a/t bases